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Previous by Date: Subject: Biological Indicators Date: Thu, 8 Jul 2010 13:00:22 -0500 Author: Beth Welmaker |
Next by Date: Subject: Re: Biological Indicators Date: Thu, 8 Jul 2010 15:04:00 -0400 Author: Kristi Ohr |
The gold standard for this protocol is Bacillus stearothermophilus endospores. This is a bacterium that only grows at temperatures above 56 degrees C (can't seem to do symbols!) Autoclaving at 121 degrees C for 15 minutes is sufficient to kill the spores. After autoclaving, you inoculate appropriate medium and incubate in a hot water bath, with a control culture of un-autoclaved spores (which should, of course, grow). I'm afraid I have no idea of pricing. Another option is autoclave tape, which reveals writing after autoclaving. You should use this with every autoclave run (it usually says "autoclaved" or "sterile.") This is good for showing which medium has been sterilized and which hasn't (assuming the medium is not compromised.) The disadvantage to the tape is that with age, the writing can appear without autoclaving. Edward J. McGrath Science Supervisor Red Clay Consolidated School District office: (302) 552-3768 -----Original Message----- From: DCHAS-L Discussion List [mailto:DCHAS-L**At_Symbol_Here**list.uvm.edu] On Behalf Of Beth Welmaker Sent: Thursday, July 08, 2010 2:00 PM To: DCHAS-L**At_Symbol_Here**LIST.UVM.EDU Subject: [DCHAS-L] Biological Indicators I am looking for a recommendation for simple Biological Indicators to verify our autoclave sterilization effectiveness. There are several vial styles to choose from. I don't want to waste money on a poor product. Thank you in advance! Beth Welmaker, CCHO Safety Officer 11350 SW Village Parkway - Third Floor Port Saint Lucie, FL=A0 34987 772-345-4826
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